Our Journey- Classified!

Wednesday, May 25, 2011

(IV) Our Gains...

As a group,
  • discuss how the project has helped to deepen your understanding/ broaden your awareness on selected subject discipline(s)
This project has taught me much more about the ways of discovering or staining cells like tissues, its correct procedures, and why we have to do those. Before this, I thought light microscope was just a simple device, but after this experience, it has allowed me to think of the complexity of different versions of microscopes and how to improve them, and want to learn more out of school!

The cell staining session was also very interesting because i never thought we could just use some dyes to stain the different organelles of the tissue to further comprehend the tissues!

(III) Our Accomplishment


Our Mission

Our outcome for this project is to learn more about the different methods and ways to stain cells in order for scientists to be able to study the cells in an easier way. There are many different ways to study the cells by using 3 we methods we learned like histochemical staining, immunohistochemical staining and also fluorescence staining. Each of these methods have a different effects like the difference between the histochemical staining and the fluorescence staining, where the histochemical staining stains both the nucleus and the cytoplasm, the fluorescence staining stains only the nucleus. The project also requires us to work with two different microscopes, the light microscope and the fluorescence microscope, where the light microscope would be use to observe cells that have been histochemical staining or immunohistochemical staining, and the fluorescence microscope would be used to observe the cells that have went through the fluorescence staining, thus this allows us to have interaction with many different types of microscopes and thus would allow us to learn more about them.

(I) Overview of Project

This project primarily concentrates on the histology and morphology of cells and for us to learn and appreciate the usefulness of the light and fluorescence microscope. The histology and morphology of the cells is simply the visual description of how the cells look using the light microscope. We will be introduced to the optical set-up of the instrument and how it works.

This topic of Staining Liver Tissue Cells is a fascinating and mind-blowing one! We did 3 amazing experiments that include Histochemical Staining, which allows will the user to identify the nucleus and cytoplasm thorough the use of dyes(Hematoxylin and Eosin, H&E Dyes), Immunohistochemical Staining, which allows the user through the use of antibodies, to locate the antigens(Eg.Proteins). The last, but not least one, is the Fluorescent Staining which allowed the user, with the help of a highly advanced Light Microscope to detect the nucleus found in the liver Tissues!

-Part 1 adapted from The Nayang Technological University: SST-NTU Flagship Programme: Project 2: Project 2 Manual.pdf

Our Team

Teng Zheng Jie S2-02
Ng Yuzhe S2-02